Method is based on unique properties of thermostable duplex-specific nuclease (DSN) from Kamchatka crab that is capable of selectively cleaving only double-stranded DNA
cDNA normalisation results in equalisation of the abundance of different transcripts and increase in the number of previously non-detected genes in a cDNA library.
This essentially increases the efficiency of transcriptome analysis and functional screening.
Each cell is known to express from about 10,000 to 60,000 genes, and transcript abundance varies from 200,000 copies to less than one copy per cell.
Dramatic difference in gene representation complicates analysis of primary cDNA libraries, especially if sequence determination is being applied for gene discovery efforts.
cDNA normalisation equalise concentration of different transcripts in cDNA population significantly reducing abundance of the most redundant ones.
Evrogen cDNA normalisation method provides unique opportunity to normalise cDNA enriched with full-length sequences.
The method is based on unique properties of thermostable duplex-specific nuclease (DSN) from Kamchatka crab that is capable of selectively cleaving only double-stranded DNA.
Normalisation is performed prior to library cloning and does not include physical separation steps.
Evrogen cDNA normalisation kits allow the preparation of normalised cDNA ready for either directional or nondirectional cloning of a cDNA library.
Besides the kits, a custom cDNA normalisation service is available.
