Virus binding capacity of ready-to-use kit for adenovirus purification, initially launched at the beginning of 2005, has been doubled while the price has remained constant
Vivascience introduces an upgraded version of the Vivapure AdenoPack 100, a ready to use kit for adenovirus purification, to the market.
AdenoPack 100 was initially launched at the beginning of 2005, and now to further increase customer benefit against traditional CsCl gradients, the virus binding capacity of the AdenoPack 100 units has been increased two-fold.
Now up to 1x10^13 virus particles can be purified from 200ml cell culture, for the same price as before.
For even more convenience, protocols are provided for optionally running the virus purification with a peristaltic pump or with an infusion pump, in additional to detailed instructions for a manual operation supplied with the kit.
This new kit still includes all reagents and devices necessary for clarification, purification and concentration of Adenovirus from HEK293 cells.
The rapid two-hour purification protocol is based on Vivascience's ion exchange membrane incorporated in AdenoPack syringe filter units.
The devices are said to be unique in their capability to efficiently capture and recover large virus particles - when compared to chromatography media, membrane adsorbers provide large 3000nm pores allowing unrestricted access and recovery of adenovirus from the charged adsorber surface.
In a fast and simple procedure up to 1x10^13 purified virus particles are recovered in a millilitre for in-vitro transfections.
In contrast to traditional caesium chloride density gradient methods, virus purification with Vivapure AdenoPack is fast, simple, not toxic to virus and cells and leads to a higher virus titer using the same amount of original cell culture, says Vivascience.
Additionally, expensive equipment as an ultracentrifuge is no longer needed.
The membrane adsorbers with porous matices, high capacities, low differential pressure, high flow rates and low unspecific adsorption are said to show excellent performance in small-scale virus purification.
However, they are also scalable to confirm to cGMP facilities to large volume, high performance separation, reducing the processing time by a factor of ten in the final process.
