This free 28 page guide from Harvard Apparatus explains the theory and practice of using equilibrium dialysis for binding studies
Equilibrium dialysis has long been accepted as the definitive way of studying binding between protein and ligand.
This guide discusses the analysis of ligand binding experiment procedures and protocols.
In addtion, it covers data linear regression, introduction to linear regression, the Scatchard plot non-linear regression, introduction to non-linear regression, and sum-of-squares analysis.
Included in the guide are product descriptions of reusable and disposable single use products such as the Dispo-Equilibrium Dialyzer, Micro-Equilibrium Dialyzer, Equilibrium Dialyzer-96, and the Plate Rotator Multi-Equilibrium Dialyzer Graphpad Prism.
Also key bibliographies providing background and comparisons of different equilibrium dialysis techniques are available.
Additional information is can be obtained from the Harvard Apparatus website, which provides bibliographies, PDF files of reference papers, procedures and additional product information.
