Syngene has introduced Dymension 3 software for rapid, yet reproducible analysis of proteins run on two-dimensional (2D) multi-stain gels, such as Cyanine dyes.
Syngene, a manufacturer of image analysis systems, has introduced Dymension 3 software for rapid, yet reproducible analysis of proteins run on two-dimensional (2D) multi-stain gels, such as Cyanine dyes.
For proteomics studies, 2D multi-stain gels are considered to be a more precise method of analysis because each protein spot has its own internal standard, which eliminates the problem of gel to gel variation.
However, aligning these gels can be a very time consuming task, says the company.
Now using Dymension 3 software, scientists can automatically overlay images of gels showing up to three contrasting (fluorescent) labelled protein samples.
Then using its spot-finding algorithm, Dymension 3 instantly locates and analyses protein spots, assigning statistical confidence to each and every difference, thus accurately highlighting specific relevant proteins.
In addition to being more accurate, Dymension 3 is also faster than any other 2D analysis software currently available because the process, from alignment and 2D image analysis, through to report generation, takes just minutes, says Syngene.
Additionally, the software can produce results so precise they need little post-image editing.
This speed of image analysis and editing means users of the software could save many valuable research hours.
Laura Sullivan, Syngene's divisional manager explained: "It is widely acknowledged that 2D multi-staining is a reproducible method of protein analysis so we are delighted to produce a system, which perfectly complements this technology".
"By harnessing the power of our Dymension 3 software to automatically analyse this type of gel, scientists can be guaranteed top quality data, giving them the confidence that what they are identifying are those all important biologically relevant proteins".
