Gentamicin determination by ion chromatography

Gentamicin is a broad spectrum water-soluble aminoglycoside antibiotic, which is produced by fermentation of micromonospora purpurea.

It is a complex mixture of four major components: gentamicin C1, C1a, C2 and C2a.

In addition, the minor component C2b is also of interest, although its concentration is very low.

A high performance liquid chromatography (HPLC) determination requires a very complex system with quaternary gradient and derivatisation of the sample prior to injection.

This very time and cost consuming, and Metrohm therefore offers a determination with a pulsed amperometric detector and isocratic elution on a polystyrene column.

The sample is injected directly into the chromatography system.

Long time derivatisationis no longer necessary.

If the sample has a very high loaded matrix, it is not a problem in a Metrohm system, says the company, to implement Misp (Metrohm inline sample preparation technique) such as inline filtration or inline dialysis.

The method is according to the European Pharmacopeia.

The MIC 9 advanced IC system combines the two most important electrochemical detection methods - conductivity measurement, and amperiometric determination.

Conductivity with the 819 high performance detector analyses either cations with electronic suppression or anions with chemical suppression down to the lowest ppb range.

The pulsed amperometric detector of the 817 Bioscan determines readily oxidisable or reducible substances.

The detection sensitivities are equal to those of the conductivity detector.

With the MIC 9 users will receive a completely flexible system, with which they can work with two channels, either in parallel or independently.

The only common system component is the 838 advanced IC ultrafiltration sample processor which, thanks to its ultrafiltration cell, is said to be outstandingly suitable for sample preparation.