Anachem has claimed that its Anachem/Gilson fraction tapping FT-LC system allows users to remove water, desalt and reformat prep HPLC fractions in less than 15 minutes.
The speed of purification and level of purity obtained with preparative high performance liquid chromatography (prep HPLC) has increased over the last 20 years, to the extent that it is no longer a bottleneck in the pharmaceutical development cycle.
However, delays have shifted downstream to the time taken to recover the purified product.
While a prep HPLC run can be completed in less than 15 minutes, it can take between eight and 24 hours to recover the purified compound, dry it, reconstitute it in the desired solvent and format it into a compound library.
This is due to the usually high water composition of the collected fraction, the potential need to change the product structure and remove compounds such as TFA, plus reformatting the compound into another solvent or structural form.
Anachem said this bottleneck can be eliminated with its FT-LC system.
The system has been developed around the Gilson Prep HPLC platform and Flowtrap from Varian and uses application specific software.
The FT-LC system can reduce the water content of fractions collected from the prep HPLC system, from 88 per cent to less than one per cent in under 15 minutes.
Included in the process is a step to automatically convert compounds from acid to base form.