Sartorius has completed its adenovirus purification product line with the introduction of the downscale kit, the Vivapure Adenopack 20
This centrifugal format kit follows the Vivapure Adenopack 100, Sartorius's first ready to use kit for adenovirus purification from 200ml cell culture medium, and the Vivapure Adenopack 500 designed to meet the great demand for the purification of adenovirus from larger cell culture volumes of up to 500ml.
Fast and easy to use kits for adenovirus purification.
Recombinant adenovirus vectors are the preferred method for a wide range of gene delivery applications.
The Adenopack adenovirus purification and concentration kits offer researchers who need to recover up to 3x10^13 purified recombinant adenovirus particles for in-vitro transfection a fast, safe and easy to use solution.
The kits include all reagents and devices necessary for clarification, purification and concentration of adenovirus type 5 from HEK293 cell cultures in only two hours.
These straight forward kits replace time-consuming and labor-intensive 48 hour CsCl density gradients.
Rapid virus purification by membrane chromatography.
The Sartorius ion exchange membrane adsorber technology used in Adenopack is unique in its capability to efficiently and rapidly capture and recover large virus particles.
When compared to chromatography media, membrane adsorbers provide large 3000nm pores allowing unrestricted access and recovery of adenovirus from the charged adsorber surface.
Convective flow through the Adenopack syringe filter devices provides high-speed separations not possible with traditional chromatography and cesium chloride density gradients.
Our membrane adsorbers with porous matrices, high capacities, low differential pressures, high flow rates and low unspecific adsorption show an excellent performance in small scale virus purification.
However, they are also scalable and confirm to cGMP facilities to large volume, high performance separation, reducing the processing time by a factor of ten in the final process.
Three different kit sizes to meet every need.
Adenopack kits are offered as Adenopack 20, Adenopack 100 and Adenopack 500, for the purification and concentration of adenovirus type 5 from 20 ml to 500 ml cell culture, leading to 1x10^11 to 3x10^13 purified viral particles.
For each sample volume, the most convenient handling method is offered for ultimate convenience.
To this end, preparations using Adenopack 20 are pursued in spin column format in a centrifuge, Adenopack 100 is a manually operated kit in syringe filter format, and Adenopack 500 is a pump driven kit.
Vivapure Adenopack 100 can optionally be operated with a laboratory pump and an infusion pump, for which protocols are provided on Sartorius's website.
Additionally, the tubes and adaptors needed for these operation modes can be ordered.