Spin columns represent a new generation of immobilised metal affinity chromatography (Imac) purification devices for use within standard centrifuges
Vivascience is continually developing ground breaking protein purification products that harness the power of membrane adsorber technology, says parent company Sartorius.
Vivapure Metal Chelate Mini spin columns for the purification of proteins with poly-histidine tags are just one of the new devices available that allow easy sample handling in simple and fast centrifugation steps.
Vivapure Metal Chelate Mini spin columns represent a new generation of immobilised metal affinity chromatography (Imac) purification devices for use within standard centrifuges.
Imac is a common and effective tool for the purification of poly-histidine tagged proteins.
The method is based on the ability of some proteins to bind to immobilised metal ions.
An especially strong interaction takes place with the commonly used poly-histidine (HiS)6 tag with six consecutive histidine residues.
Using the Imac principle, poly-histidine tagged proteins can be concentrated to a high degree of purity, even from cell lysates or culture supernatants.
The Vivapure Metal Chelate Mini spin columns have covalently bound IDA (iminodiacetics acids) groups on the membrane.
The IDA groups can be loaded with different metal ions depending on the particular application.
Sartorius Vivascience suggests using nickel (Ni**) cobalt (CO**), copper (Cu**) or zinc (Zn**) ions, but also different metal ions can be easily immobilised on the membrane. Proteins engineered with poly-histidine tags passing through the prepared membrane are preferentially bound.
These bound proteins can be easily eluted from the membrane using buffers with varying concentrations of imidazole.
The Vivapure Metal Chelate spin columns can be used in conjunction with Vivaspin 2 or 4 that are specifically designed for rapid protein concentration.