The Kapa Biosystems SYBR Fast qPCR Kit contains a novel DNA polymerase evolved specifically for real-time PCR.
The reaction efficiency of qPCR is critical for accurate and reliable gene quantification.
Wild type Taq DNA polymerase is often inhibited by real-time PCR reaction conditions, leading to significant loss in specificity and sensitivity of the assay.
Kapa SYBR Fast qPCR Master Mix contains a novel DNA polymerase engineered to perform optimally in stringent qPCR reaction conditions.
It exhibits dramatic improvements in signal-to-noise ratio (fluorescence), cycle threshold (Ct), linearity and sensitivity.
The novel DNA polymerase and proprietary buffer system enhances the amplification efficiency of difficult templates, including GC and AT-rich templates.
The kits are suited for gene-expression analysis, microarray validation, low copy gene detection and gene knockdown validation.
Kapa SYBR Fast qPCR is available in a compatible formulation for all real-time PCR machines.
These kits remove the need for lengthy re-optimisation.