Kapa2G Robust DNA Polymerase is a versatile enzyme evolved from Taq DNA polymerase, available in the UK and Ireland from Anachem.
The company said the novel amino acid mutations in Kapa2G Robust DNA Polymerase offer higher processing capabilities and specific activity.
They ensure robust performance across a range of template and amplicon types and offer improved tolerance to common PCR inhibitors.
The result is higher yield and sensitivity per unit of enzyme.
All Kapa2G Robust kits are supplied with three buffers and one proprietary enhancer that offer extended optimisation options for difficult amplicons or templates.
Like wild-type Taq, Kapa2G Robust DNA Polymerase has a 5'-3' polymerase and exonuclease activities but no 3'-5' exonuclease (proofreading activity).
The fidelity of Kapa2G Robust DNA Polymerase is the same as that of wild-type Taq.
Kapa2G Robust PCR kits are designed for the amplification of DNA fragments up to 5kb in standard end-point PCR assays from a variety of templates.
The kits are suitable for amplification from templates with a high GC- or AT- content, templates containing common PCR inhibitors (salts, urea, SDS, ethanol or EDTA, for example) at levels inhibitory to wild-type Taq, and optimisation of low-yield or low-specificity assays.
Anachem said that with Kapa2G Robust PCR kits, 100 per cent success rate in Colony PCR is achievable from plated bacterial colonies or overnight cultures.
Amplicons generated with the Kapa2G Robust PCR kit are suitable for routine downstream applications, including restriction enzyme digestion, cloning and sequencing.