Oxford Expression Technologies (OET), a provider of baculovirus-based protein expression products and services, has launched a range of transfer plasmids with signal sequences and promoters.
These include pOET-1TM and pOET-1NTM and increase cloning efficiency and expression of foreign genes.
OET's transfer plasmids and its existing expression suite of FlashBACTM products (including FlashBACGOLDTM, FlashBAC10TM and FlashBACULTRATM) will be available directly from the company and a number of newly appointed distributors, which are based in Canada, the US, Scandinavia, Italy, the Netherlands, Japan, India, China, Taiwan, Singapore and South Korea.
The pOET transfer plasmids have been designed to be smaller for improved cloning efficiency and ease of use.
Both pOET-1 plasmids contain a large multiple cloning site (MCS), allowing foreign genes to be inserted in the correct orientation.
AcMNPV sequences flanking the gene in the transfer plasmid's MCS allow recombination with the FlashBACTM DNA to insert the expression cassette into the polyhedron (polh) locus under the control of the polh promoter.
Additionally, pOET-1NTM has been designed for the high-level expression of foreign genes fused to an N-terminal 6xHis tag, easing the purification of the recombinant protein using Ni-NTA Agarose.
James Bernard, chief executive officer of OET, said: 'pOET-1 is compatible with any baculovirus system that utilises homologous recombination in insect cells and will enable researchers and scientists to express high levels of foreign genes.
'Our expanded distributor network will allow OET to reach a broader customer base and provide increased support for existing clients, who will be able to purchase their products more quickly and with reduced shipping costs and transportation time,' he added.
