KAPA Library Quantification Kits utilise Quantitative PCR (QPCR) to provide customers with an accurate and sensitive method for quantifying Next Generation Sequencing (NGS) libraries.
QPCR is said to be a highly sensitive approach to quantifying NGS libraries, using a minimal amount of material and eliminating the need for titrations.
It specifically quantifies only PCR-competent DNA molecules, allows for the accurate quantification of low-concentration libraries and significantly reduces cluster-density variations.
It can also be automated for high-throughput applications.
The KAPA Library Quantification kits are optimised for the Illumina Genome Analyzer, Roche 454 Titanium series and Roche 454 FLX series platforms.
They include defined, reliable DNA-concentration standards and QPCR reagents, containing a DNA polymerase engineered for SYBR Green-based QPCR through a process of molecular evolution.