The Acquity UPLC system with 2D technology from Waters is suitable for complex samples including food, impurity profiling and confirmation, bio-analysis and biopharmaceutical applications.
Designed for chromatographers, 2D technology for Acquity UPLC systems gives separation scientists added resolving power, particularly for samples that are hard to resolve by conventional means, while offering greater sensitivity and selectivity.
Based on Waters' established Acquity UPLC technology, the Acquity UPLC system with 2D technology gives scientists a capability for trapping, heart cutting and parallel column regeneration to eliminate matrix effects, increase the overall speed of analysis, enhance assay ruggedness, increase selectivity and sensitivity and use MS-incompatible solvents for the first dimension of separation.
Research organisation PPD routinely employs 2D chromatography for separations demanding extra speed, separations power and sensitivity.
Rand Jenkins, scientific director of chromatography sciences at PPD, said: 'We have a total of 54 LC/MS systems and most are set up to run 2D chromatography.
'Eighty per cent of the time, our scientists use a simple 2D approach to remove residual lipids from the sample matrix,' he added.
For more demanding assays requiring maximum selectivity and sensitivity, PPD scientists employ 2D heart-cutting techniques using two analytical columns to isolate the analyte peaks of interest.
'The ability to perform 2D UPLC separations using Waters technology has enabled some of our most challenging assays that require ultra-high sensitivity while maintaining excellent ruggedness and instrument up-time,' continued Jenkins.
The Waters Acquity UPLC system with 2D technology features Masslynx software for operational control and data management.
It is a compatible front-end to Waters' Synapt- and Xevo-brand mass spectrometers and quadrupole mass detectors.
Waters is offering its present Acquity UPLC customers a path to upgrade their systems to enable 2D separations with the addition of several hardware components and new software.
According to the company, 2D chromatography is a valuable strategy for resolving individual sample constituents that are not easily detected or quantified owing to the presence of other closely related or co-eluting peaks.