Fluidigm has introduced the 192.24 Dynamic Array integrated fluidic circuit (IFC), which is suitable for use in agricultural biotechnology and genome-wide association studies.
The 192.24 IFC is designed to genotype 192 samples against 24 SNP assays in a single run, providing 4,608 data points in just one hour.
The IFC features a design that increases sample throughput for common SNP genotyping applications.
Using the new Fluidigm IFC RX controller, the new Dynamic Array IFC enables sample loading within 30 minutes and thermal cycling in another 30 minutes, resulting in an accelerated workflow.
The 192.24 Dynamic Array chip addresses the high-throughput needs of genotyping by providing a fourfold increase in sample throughput when compared with the 48.48 Dynamic Array IFC.
This accelerated workflow and increased throughput enables more data points, while the 192.24 chip retains Fluidigm's 99.75 per cent or greater call accuracy and 99 per cent or greater call rates.
The new chip can be read using either the Biomark HD or EP1 system.
Gajus Worthington, Fluidigm president and chief executive officer, said: 'The Fluidigm 192.24 Dynamic Array chip enables life science researchers to achieve ultra-low cost and rapid throughput while maintaining full assay and reagent flexibility.
'Providing the ability to use fewer SNP assays and run a high number of samples is a format that is ideal for some applications in ag-bio and for GWAS follow-up studies,' added Worthington.
While the 192.24 Dynamic Array chip will support all industry-standard assays, SNP-type assays offer a low-cost SNP genotyping solution, are made on demand and are ready to meet high-throughput needs for a wide range of agricultural applications and the confirmation of next-generation sequencing results.
Fluidigm IFCs, including the 192.24 chip, use just microlitres of materials, allowing scientists to achieve comprehensive genetic profiling from miniscule amounts of sample.
Each reaction chamber in the 192.24 chip requires only eight nanolitres of material.
In addition, all Fluidigm Dynamic Array IFCs feature a matrix-style architecture that automatically assembles every possible reaction between the samples and the assays, providing up to 99 per cent fewer pipetting steps than needed when using 384-well plates.
