Culture media for the detection of Enterobacteriaceae
Violet Red Bile Glucose Agar (VRBGA) delivers Enterobacteriaceae counts after 18-24 hours incubation.
The medium is used widely in the food and dairy industries as part of critical hygiene monitoring procedures.
Since glucose is fermented by all members of the Enterobacteriaceae, VRBGA enables the growth of all the key organisms of interest including E. coli, Salmonella and Shigella species.
The inclusion of bile salts and crystal violet in the medium inhibits Gram positive and non-enteric organisms, while an overlay procedure ensures anaerobic conditions, suppressing the growth of non-fermentative Gram negative bacteria.
Following incubation, the number of red/purple colonies is counted, allowing calculation of the number of Enterobacteriaceae in the original sample.