The small RNA labelling kit exploits the true power of the ULS labelling technology in its ability to directly label any and all sizes of naturally occurring nucleic acids without the need of enzymes
Kreatech Biotechnology announces the release of the latest in a continuing line of labelling kits for gene expression applications, the Small RNA Labelling Kit.
The small RNA labelling kit exploits the true power of the ULS labelling technology in its ability to directly label any and all sizes of naturally occurring nucleic acids without the need of enzymes.
The discovery of small RNAs that have regulatory effects on gene expression has excited researchers to elucidate and characterise the function of these molecules in molecular processes and as possible tools in drug discovery.
One common way to examine these small RNAs, either siRNAs or miRNAs, is to analyse them on a microarray.
Due to their small size, these RNA molecules prove very difficult to label via the enzymatic reactions common to NHS-ester chemistry.
Since the ULS labeling technology it is a non enzymatic labeling reaction whose labelling efficiency is independent of fragment size, ULS enjoys a unique advantage because it can just as efficiently label small RNA molecules as it does all nucleic acids.
The small RNA labeling kit labels small RNAs such as miRNA or siRNA within 15 minutes in a one step incubation step, without enzymes.
This kit will provide users with the reagents necessary to label 20microg of small RNA as well as the specially designed Kreapure purification columns.
These columns were developed to specifically remove unreacted ULS reagent from the labeling reaction.
The Kreapure columns have no affinity for nucleic acids and for this reason the recovery is extremely high and fragments of all sizes flow through, making it ideal for work with small RNA samples.
This kit offers the ULS labeling molecule bound to leading dyes from GE Healthcare/Amersham (CyDyes).
"Our Small RNA labeling kit expands and showcases the versatility of the ULS technology.
"This kit allows for the fast, robust and reproducible labeling of miRNAs and siRNAs, important emerging research tools, where other enzymatic methods have failed.
"Additionally, ULS now proves that is truly a 'molecular glue' that is independent of fragment length, is platform independent, substrate independent, and will enable the researcher to broaden the scope of their experimental conditions", according to Brent Keller, general manager of Kreatech USA and VP Commercial Operations.
Kreatech's ULS technology relies on the special binding properties of transition metals, especially platinum, to form coordinative bonds with biomolecules by binding to specific sites on DNA, RNA and proteins.
In this way, ULS acts as relatively simple 'molecular glue' for DNA, RNA and proteins.
As a result of its versatility, customers have a choice of the molecular label attached to the ULS they can use to label the biomolecules of interest.
Kreatech currently commercialises labelling kits for aRNA labelling, small RNA labelling, arrayCGH labelling, general nucleic acid labelling, and recently introduced kits for protein labelling on antibody arrays.
Kreatech Biotechnology, a private company founded in 1990, focuses on the creation, development, production and marketing of innovative diagnostic and life science products.
Kreatech's proprietary Universal Linkage System (ULS) offers a reliable, robust and easy to use non-enzymatic technology that can be applied to a broad range of biomolecules and labeling agents.
Kreatech says it is committed to achieve and maintain the highest standards in production and quality control as well as in the relationships with customers and partners.
The company's quality policies are demonstrated by the implementation of ISO 9001 (2000) and ISO 13485 (GMP 2003) guidelines.
