Spectral reassignment software provides a means of separating overlapping fluorophores, resulting in accurate and better quality images
Bio-Rad now offers spectral reassignment software on its latest and most affordable confocal imaging systems - CellMap.
Expanding the cell science product portfolio are the latest confocal systems CellMap ID and CellMap IC - the first two models in a new family of products designed to produce high quality confocal images at an affordable price for individual laboratories.
With its fast sequential scanning image acquisition and based exclusively on solid state lasers, CellMap is designed for individuals who want dual colour imaging.
CellMap ID is designed to image blue and green fluorophores, and CellMap IC is designed for imaging green and red fluorophores.
Due to CellMap's design, users can image up to three fluorophores using only two laser sources and one detector.
These two systems enable users to choose equipment purposely developed for their needs and offer a wealth of features to improve speed, ease and quality of their research.
Based on the technology of Bio-Rad's high end confocal systems, CellMap now offers spectral reassignment as a software option.
Spectral reassignment provides a means of separating overlapping fluorophores, resulting in accurate and better quality images.
Spectral reassignment may be used in cases of bleed-through or where the use of overlapping fluorophores cannot be avoided.
Although not required for routine use with the CellMap, it offers the option of collecting and separating more than two fluorophores.
With CellMap ID, DAPI, FTIC and TRITC can be collected and FTIC and TRITC separated using this software. With the CellMap IC, two red fluorophores such as propidium iIodide and TRITC can be used along with FITC, and the software will separate the two red fluorophores.
Example images are available.