Tecan's Quad4 technology, featured in Infinite M200 and Safire2 microplate readers, blocks stray light by a factor of a million more than typical dual monochromator configurations
Unwanted stray light and the resulting background noise are the most critical factors affecting performance and sensitivity of microplate readers.
Monochromators typically reduce undesired wavelengths by a factor of a thousand, so using four monochromators instead of the usual two blocks significantly more stray light and reduces background noise while enabling full wavelength flexibility.
The Quad4 technology consists of two monochromators for excitation and two monochromators for emission, connected in series.
Using four monochromators gives a true spectral scan with none of the artificial peak shifts that can be caused by using cut-off filters combined with a monochromator, resulting in incorrect peak maximums.
The unique design of the monochromator concept allows the microplate readers to perform fluorescence scans up to six times faster than comparable instruments that contain two monochromators combined with cut-off filters.
The Quad4 technology provides consistent performance over the whole wavelength spectrum and you can tune in any wavelength without having to optimise filters.
