At a recent conference in Japan, Kouhei Shiba, from Sysmex, used zeta potential data generated with the Malvern Zetasizer Nano to demonstrate a new method for evaluating the quality of protein samples
At the recent Joint Conference of the Asian Crystallographic Association and the Crystallographic Society of Japan (Tsukuba, Japan; 20-23 November 2006 ), Kouhei Shiba, from Sysmex, used zeta potential data generated with the Malvern Zetasizer Nano to demonstrate a new method for evaluating the quality of protein samples.
NMR and X-ray crystallography are successfully used techniques for protein structure analysis.
With both methods sample preparation is now the most demanding step; the requirement for crystals for X-ray is a particular challenge.
Data produced recently indicate a link between zeta potential and the quality of protein samples and associated crystals.
This link affords researchers an opportunity to optimise the sample preparation process.
Zeta potential is a measure of the forces of attraction/repulsion at the boundary of a particle.
The Zetasizer Nano uses M3-Pals technology to rapidly deliver accurate zeta potential measurements for either aqueous or non-aqueous systems.
With molecular weight and particle size measurement capabilities, automated sample preparation and user-friendly software the instrument is proving a powerful and useful tool for protein researchers.