Researchers in the Netherlands use new technology from Applied Biosystems to identify different expression patterns of microRNA molecules in normal and cancerous cells
Scientists at the Erasmus MC-University Medical Center in Rotterdam, Netherlands and Applied Biosystems say they have made significant advances in testicular cancer research by identifying and analysing a set of specific microRNA molecular markers that are involved in the development of testicular tumors.
The study, published in the November 2007 issue of the Journal of Pathology (vol 213 issue 3), provides new information about the unique cellular events that cause testicular cancer.
These findings could potentially lead to earlier identification of the disease and new approaches for treating the cancer.
Although testicular cancer is relatively rare in the general population, it is the most common cancer in men between the ages of 15 and 44 years old.
This study has found that normal and cancerous cells contain distinctly different amounts of molecules called microRNAs.
The findings also showed that a tumor's microRNA expression pattern provides vital information about the malignancy of the tumour.
This new information could help clinicians to identify testicular cancer patients more quickly and more accurately, and provide more precise prognoses than current approaches.
MicroRNAs are short non-coding RNA molecules that regulate gene expression.
They play an important role in many biological processes, particularly in cell differentiation and development.
MicroRNAs have also been implicated in a number of different diseases, including various cancers.
Research related to microRNAs is one of the fastest growing areas in biology.
The researchers in this study, who included Prof Leendert Looijenga, group leader within the department of pathology at the Erasmus Medical Center, which is affiliated with the Josephine Nefkens Institute in Rotterdam, with Simone Gunther, and Jon Sherlock from Applied Biosystems, profiled the expression patterns of 157 microRNA molecules in a series of different testicular tumours and normal testicular tissue samples.
To conduct this research, the scientific team deployed a high-throughput, quantitative PCR-based approach to compare the microRNA expression profiles of multiple cell tumour samples in parallel.
The real-time PCR workflow comprised an Applied Biosystems 7900HT real-time PCR system, running a variety of chemistries that included Applied Biosystems TaqMan MicroRNA assays configured in a panel format, and Applied Biosystems TaqMan Array human microRNA panel.
The use of this real-time PCR workflow enabled the researchers to accurately determine the relative levels of mature microRNAs across a large number of different tumor samples, including those microRNAs present at levels too low to detect and quantify using other methods.
The large dynamic range of the TaqMan Assays allowed researchers to measure, in parallel, a broad range of target microRNA levels.
The RT-qPCR data were analysed using Real-Time StatMiner, a data analysis software tool designed by Integromics in collaboration with Applied Biosystems.
"These findings have provided us with a new level of information for understanding the biology of cancer and these will also be applicable to breast, lung, colon and other cancers," said Prof Leendert Looijenga, group leader within the department of pathology at the Erasmus Medical Center.
"The contribution from Applied Biosystems in this study was particularly important in helping us to interpret our findings in the relevant biological context, and the accuracy and sensitivity of the TaqMan chemistries gave us great confidence in our results."
