Fast Sybr Green master mix provides life scientists with a new choice in fast-enabled PCR chemistries that is expected to deliver results in less than half the time of standard Sybr green reagents
Applied Biosystems has introduced a new master mix reagent designed to accelerate real-time PCR (polymerase chain reaction).
The company says its Fast Sybr Green master mix establishes it as the only provider of complete workflow solutions for fast real-time PCR that include a portfolio of fast-enabled reagents, instruments, and other consumables.
Real-time PCR, which is well established in academic institutions as well as the biotechnology, pharmaceutical and medical research industries, is a laboratory method used to simultaneously detect and determine the amount of nucleic acids present in samples.
The resulting data help life scientists to more precisely assess how changes in the amounts of DNA or RNA targets contribute to the development of disease or how they relate to biological processes in cells.
Life scientists seeking to increase the throughput of their real-time PCR workflows and deliver results faster often adopt 'fast' PCR.
Fast real-time PCR allows researchers to conduct real-time PCR experiments in less time by using an integrated system of optimised instruments and specialised chemistries that enable faster reaction times without compromising data quality.
Fast Sybr Green master mix was designed to accelerate the performance of real-time PCR while maintaining a high level of data quality by providing the complete mix of specialised chemistries necessary for fast-enabled Sybr Green-based real-time PCR experiments.
Sybr Green dye is a cost-effective and easy to use nucleic acid labelling method for real-time PCR, which is commonly used in applications such as gene expression, chromatin immunoprecipitation (ChIP) analysis, and microarray validation.
Scientists at the Segal Cancer Center and the Lady Davis Institute at the Mortimer Davis Jewish General Hospital affiliated with McGill University are successfully using the Fast Sybr Green master mix as part of a fast real-time PCR workflow to study epigenetic and arsenic-based chemotherapy for leukaemia and solid tumours.
These experiments generate gene expression and ChIP data that are important for advancing their research.
"The use of real-time PCR is vital to the research we are conducting and the more we can do to cost-effectively increase throughput, without compromising the quality of results, the better equipped we will be to advance our work," said Koren Mann, a project director at the Segal Cancer Center.
"Fast Sybr Green master mix provides a fast alternative with a familiar workflow that allows our researchers to easily integrate it into their existing real-time PCR applications".
"Fast Sybr Green master mix should allow us to achieve higher throughput while also maintaining the high level of sensitivity and specificity required for our gene expression and ChIP analysis research".
Fast Sybr Green master mix achieves its increased performance by using a specialised formulation that includes a proprietary enzyme system manufactured by Applied Biosystems to perform faster PCR experiments without compromising the quantitative results.
These highly purified enzymes minimise non-specific product formation, which increases the efficiency of amplifying targets and, ultimately, the accuracy of the data.
These enzymes are combined with all the necessary components - excluding template, primers, and probes - to form a master mix that is capable of decreasing the PCR run time by at least 60percent on fast-enabled real-time PCR instruments.
"Fast Sybr Green master mix expands our portfolio of consumables for real-time PCR and provides our customers with a choice of chemistries that are tailored to their specific applications," said Peter Dansky, president for Applied Biosystems's molecular and cell biology consumables division.
"Integrating these specialised master mixes with our industry leading real-time PCR instrument systems enables our customers to perform fast real-time PCR experiments that accelerate their research in gene expression, ChIP analysis and microarray validation, among others."
