This four-page bulletin demonstrates how varying multiple parameters affect delivery of either siRNA or plasmid DNA into human primary fibroblasts (HPF) cells
Bio-Rad Laboratories's new product guide (bulletin 5603), describes how researchers can optimise transfections in mammalian cells using its new Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer.
Results of the experiments clearly demonstrate increased transfection efficiency as measured by real time Q-PCR of specific gene knockdowns or increased protein expression by luciferase assays.
While many parameters can affect electroporation, with the Gene Pulser MXcell system optimization of such parameters can be accomplished in a multiplexed manner easily and rapidly in a single experiment.
Availability.
Bulletin 5603 is available either from a local Bio-Rad sales office or it may be downloaded.
For more information on optimisation of electroporation parameters and conditions see also Bio-Rad's technical note 5622.
