AnaSpec has introduced a new series of SensoLyte Cathepsin D Activity Assay Kits to address the issue of autofluorescence interference.
While the detection of cathepsin activity plays a crucial role in the research of a number of pathological processes, the sensitivity of traditional cathepsin assay kits has been significantly hampered by autofluorescence interference at lower wavelengths.
The SensoLyte 520 Cathepsin D activity assay kit offers Cathepsin D detection at the industry's longest wavelength.
Leveraging AnaSpec's proprietary fluorescence technology, the kit incorporates a Fret substrate that fluoresces at a much higher wavelength than standard substrates.
The higher wavelength fluorescence results in cathepsin detection that avoids autofluorescence interference from cell components and test compounds.
The Fret substrate in the SensoLyte 520 utilises a unique 5-FAM/QXL520 Fret pair (Ex/Em=490/520nm upon cleavage).
The sequence of the Fret substrate is derived from the cleavage site of Cathepsin D.
In the intact Fret peptides the fluorescence of donors is quenched by acceptors.
When active Cathepsin D cleaves Fret substrate into two separate fragments, the fluorescence of the donor is recovered, and can be monitored.
AnaSpec also offers the SensoLyte 390 Cathepsin D activity assay kit that uses a traditional Mc-Ala/Dnp Fret peptide for measurement of enzyme activity (Ex/Em=330/390 nm upon cleavage) Cathepsin is a protease that breaks apart other proteins found in many types of cells.
There are at least 12 members of the cathepsin family which are distinguished by their structure and the proteins that they cleave.
Cathepsin D is the lysosomal aspartic proteinase implicated in intracellular protein degradation.
