ABsolute Fast QPCR Master Mixes have been specially developed for Fast QPCR protocols to deliver high performance QPCR results on all major QPCR platforms in a fraction of the time previously required
Thermo Fisher Scientific announces the launch of ABsolute Fast QPCR Master Mixes for the rapid and reliable probe-based detection of DNA and cDNA targets.
By minimising QPCR protocol time1, ABsolute Fast QPCR Master Mixes help to maximise throughput while delivering unbeatable reproducibility, sensitivity and specificity.
The mixes contain Thermo-Fast DNA Polymerase, which can be activated in just five minutes at 95C (compared to 15 minutes required for traditional QPCR master mixes).
Further time savings are achieved by optimising the temperature of the combined annealing and extension step, and significantly reducing dwell times at each temperature, allowing the fast QPCR protocol to be completed in less than one hour.
ABsolute Fast QPCR Master Mixes improve the performance of fast QPCR reactions when compared to other leading fast QPCR mixes, demonstrating increased sensitivity (lower Ct values), superior reproducibility of replicates and a more linear dynamic range.
ABsolute Fast QPCR Master Mixes are compatible with any sequence-specific probe.
In addition to accelerating QPCR analysis of DNA, the master mixes can also be used for QRT-PCR analysis of RNA targets and are ideal for use downstream of the Thermo Scientific Verso cDNA synthesis kit or any other reverse transcriptase kit.
ABsolute Fast QPCR Master Mixes are available in a broad range of formats, individually optimised to deliver fast results on all major QPCR platforms, both standard and fast.
These ready-to-use reagents also contain the popular ABsolute blue dye.
This inert dye provides visual confirmation of accurate pipetting, ideal for use with white QPCR plates, which reflect increased signal back to the detector for improved assay sensitivity.
The features of ABsolute Fast QPCR Master Mixes deliver QPCR results in a shorter time, with increased confidence in reproducibility, sensitivity and specificity.
