With its Prime Pro 48 quantitative real-time polymerase chain reaction (qPCR) machine, Techne claims to have produced the fastest run time of any block-based qPCR system currently available.
Techne also says its machine has the highest thermal block uniformity; uniformity that allows researchers to use duplicates, where otherwise triplicates might be required.
The key feature with qPCR is that the amplified DNA is detected as the reaction progresses - that is in “real time” - as opposed to the older standard PCR, where the product of the reaction is detected at the end.
As the Prime Pro 48 is such a high specification qPCR machine with a unique and economical 48-well PCR plate, it is ideal for a number of different applications including:
phytopathogen identification e.g. agriculture
genetically modified organisms detection
diagnostics e.g. infectious disease and cancer
microbiology e.g. food safety and water quality
clinical quantification and genotyping e.g. virus identification
With PCR, efficiency is a product of temperature control.
The more precise the control during the denaturation and annealing steps the better and the greater the temperature uniformity across the plate the more likely you are to have equal sample processing.
The Prime Pro 48 has a unique heating/cooling mechanism that can achieve, within a fraction of a second, ±0.1°C of dwell temperature in each block well.
This helps enable the Prime Pro 48 to achieve 40 cycles in 40 minutes (before optimisation) and 40 cycles in 15 minutes after optimisation.
This means you can genotype in four minutes, with 99% accuracy and set up a 4plex reaction in only four mouse clicks. The small plate also cuts reagent costs in half, but still produces a strong fluorescence signal.
The Prime Pro 48 is fully MIQR compliant and consistent as well.
This makes it possible to compile data from different experiments and collate results from different instruments and even different laboratories.
The adaptive LED Control eliminates the uncertainty of saturated unknowns, plus there is no light ’bleaching’.