Nikon Instruments has enhanced its A1 series of confocal laser scanner systems with the launch of the A1SI spectral detector option.
The A1SI option provides 32-channel spectral imaging with speeds of to 16fps at 512 x 64 pixels and real-time spectral unmixing.
The system reduces light exposure during multiprobe studies, capturing full spectral data in one scan.
Coupled with high-speed spectral imaging, a clearly resolved image with no crosstalk between closely overlapping fluorophore emissions can be created in real time.
In addition, a virtual filter function freely utilises the 32 channels, providing the flexibility to handle any new fluorescence probes.
Desired spectral ranges can be selected and the total intensity of each range adjusted individually.
Broader band spectral imaging is achieved on the A1SI, which requires only one pass of the laser to capture all the spectral data in the sample.