Bio-Rad Laboratories has announced the availability of a technical note (tech note 5782) entitled 'In-Gel Protein Quantitation Using the Criterion Stain Free Gel Imaging System'.
Bio-Rad's Criterion Stain Free system is a protein visualisation system that utilises the standard conventions of SDS-page.
Consisting of a new formulation of Bio-Rad's Criterion pre-cast gels, a Criterion Stain Free imager and Image Lab software, the Criterion Stain Free system triggers protein fluorescence and generates a digital image of the gel after electrophoresis in two and a half minutes.
Maximum sensitivity is reached in five minutes, with equal or better sensitivity than that of Coomassie staining.
The in-depth technical note offers a comparison of standard SDS-page/Coomassie staining and Criterion Stain Free system workflows, showing a 70 per cent reduction in experiment time with the Criterion Stain Free system.
Rinsing, staining and destaining steps are eliminated from the workflow when using the system, and the imaging and analysis steps are automated, thus reducing post-electrophoresis time from more than two hours to about two and a half minutes.
The technical note (tech note 5782) is available either from a local Bio-Rad sales office or it may be requested by visiting the Bio-Rad website.
