Researchers have used Ludesi's 2D gel image and data analysis to discover potential therapeutic targets for melanoma tumours.
The researchers, at the University of Pennsylvania, Philadelphia; the National Eye Institute at National Institutes of Health in Bethesda, Maryland; and the University of North Carolina, Chapel Hill, performed kinetic proteomics of melanoma tumours as they progressed in vivo to better understand the complex interactions between the tumours and their native environments.
Proteins from the tumours were separated on 2D gels and the resulting images were sent for Ludesi to analyse using its proprietary image analysis system.
Univariate and multivariate statistical tests identified trends and co-regulation in protein expression.
The results were validated by immunohistochemistry and immunoblots.
An important finding in the study was the dynamic nature of melanomas which highlighted the need to study the disease progression at multiple time points for biomarker and therapeutic target discovery.
The researchers also identified several potential therapeutic targets for cancer therapy.
