Olympus has brought out the Fluoview FV1000-MPE multiphoton laser scanning microscope systems for deeper observation and imaging in living specimens.
Fourteen models and configurations are available.
The systems are suitable for neuroscientists and biologists wanting to image dynamic processes up to several hundred microns deep in living specimens, with minimal bleaching or damage.
Most of the systems rely on an optimally tuned custom-designed Spectra-Physics Deepsee laser, which reduces the need for higher laser power at the sample by compensating for group velocity dispersion.
The systems can handle applications using multiple spatial points or regions of interest and imaging using high-speed toggling on/off of the laser with an acousto-optic modulator (AOM).
All of the FV1000-MPE systems can be used for repeated, long-range or time-lapse exposures; infrared (IR) stimulation and imaging are available on some of the versions.
High-performance UIS2 and specific multiphoton objectives are available, including dipping lenses and IR-corrected optics.
Just two alignment points are required for set-up.
The systems are built around Olympus' FV1000 confocal imaging platform with proprietary SIM scanner.
This allows two lasers to be used simultaneously in different regions of the specimen.
When combined with multiphoton capability, the microscopes can use two multiphoton lasers simultaneously for 3D uncaging experiments.
