Invitrogen has released the Premo Halide Sensor for functional studies of ligand- and voltage-gated chloride channels.
Chloride channels are involved in a variety of important physiological functions and chloride channel defects underlie a number of chronic disease states such as cystic fibrosis.
The Premo Halide Sensor is a precise high-throughput tool that allows screening for potential therapeutic agents.
The sensor is based on a halide-sensitive form of Venus, the brightest available yellow fluorescent protein.
Users simply need to introduce the Premo Halide Sensor into cells of interest 24 hours ahead of the assay.
They then need to add iodide-containing buffer to the culture, followed by test compounds.
With a simple mix and read format, a quantitative measurement of channel activity is obtained, allowing identification of compounds that modulate chloride-channel activity.
