Olympus has released a 25x NA 1.05 water immersion (WI) objective for the Olympus Fluoview FV1000-MPE multiphoton microscope system.
The objective offers a wide field of view, flexibility for patch clamping and cover slip use, outstanding infrared (IR) performance and a deep-imaging correction collar for imaging far below the surface of living specimens.
The objective has been designed for two-photon applications.
It is designed so the exit pupil diameter is completely filled with the scanned excitation laser beam.
This brightens fluorescence excitation and gives more efficient collection, enhancing instrument sensitivity and signal-to-noise ratios.
New optical coatings allow the objective to provide outstanding performance in the IR range, with up to 82 per cent transmittance from 400 to 1000nm.
The objective has a working distance of 2mm and a 35-degree angle for patch clamping.
The objective is excellent for neurophysiology and related applications.
It has a wide 27.5 FOV.
The objective is designed for deep-specimen imaging, delivers maximum detection efficiency and collects usable signal from hundreds of microns below the surface.
A correction collar is available to compensate for the refractive index mismatch that can occur between the dipping medium (water, at n=1.3) and the specimen (at about n= 1.36-1.4), giving resolution more than 500 microns deep, depending on the specimen.
