96 well plates eliminate the tedious chromatographic steps normally associated with immobilised metal affinity and ion exchange chromatography
Sartorius Vivascience announces the release of its new Vivawell 96 well plates with ion exchange membranes and metal chelate membranes.
These plates are ideal tools for developing methodologies to screen target proteins against different loading/eluting conditions and membrane types.
The Vivawell 96 well plates eliminate the tedious chromatographic steps normally associated with immobilised metal affinity and ion exchange chromatography, and are ideal for increased throughput and robotic applications.
Vivawell-96 IEX multiwell plates use the basic principle of ion exchange: separation accomplished on the basis of charges carried by solvent molecules.
This is achieved using a centrifuge with a swing-out multiwell plate rotor or vacuum manifold equipped to take standard footprint deep-well plates.
Available in four different membrane functionalities, the Vivawell-96 IEX covers every ion exchange application; strong acidic cation and strong basic anion exchanger, or alternatively weak acidic cation and weak basic anion exchanger.
Vivawell-96 metal chelate plates can quickly purify small amounts of recombinant proteins engineered with poly-histidine, (HiS)6 tag through immobilised metal affinity chromatography (Imac).
The Vivawell-96 metal chelate has covalently bound IDA (iminodiacetic acid) groups on the membrane.
These IDA groups are first charged with metal ions, eg nickel, which has an affinity for protein with complex forming amino acids residues, especially histidine, exposed on the protein surface.
Other metals that can be used include cobalt, copper and zinc.
Proteins engineered with poly-histidine tags passing through the prepared membrane are preferentially bound.
These bound proteins can be easily eluted from the membrane using buffers with varying concentrations of imidazole.
The Vivawell-96 plates are truly scaleable and supported by Vivapure metal chelate and Vivapure IEX spin columns but also fully validated large-scale membrane based chromatography systems.
