Stempro Accutase Cell Dissociation Reagent is a marine origin enzyme with both protease and collagenolytic activity.
Each lot of Accutase is individually formulated to meet quality-control standards for effective cell detachment for a variety of primary and stem-cell lines and tissues.
Accutase performs well in detaching cells for: analysis of cell-surface markers; virus growth assays; quiescence assays by serum starvation; transformation assays by oncogene transfection; neural crest cell migration assays; cell proliferation; cell haptotaxsis; tumour cell migration assays; routine cell passage; production scale-up (bioreactor); and flow cytometry.
Accutase is supplied in Dulbecco's PBS containing O.5mM EDTA4Na and phenol red and is available in a ready-to-use 100ml bottle.
Standardised protocols have been developed for human embryonic and neural stem cells.
