Febit has launched Hybselect, an automated microfluidic system for sequence-specific capture.
It targets genomic hot-spots ranging between tens and thousands of kilobases, and integrated Hybselect automation only requires 30 minutes of hands-on time.
In tests, Hybselect has captured 1,000 500 bp regions with a centrally located SNP from a reference sample.
After next-generation sequencing (NGS), the average depth of coverage for the individual 500 bp regions was 469-fold.
91.2 per cent of the 1,000 SNP positions were covered at more than or equal to 20x, and 99 per cent of those were concordant with the reference sequence, as is consistent with baseline Illumina NGS SNP calling accuracy.
98.1 per cent of the heterozygous SNP positions were detected and called correctly with no evidence of allelic bias.
