Epigentek has released the Supersense methylated DNA quantification kit to complete its line of methylated DNA quantification products.
The Supersense kit fluorometrically measures global DNA methylation shifts.
The tool complements the Methylamp Global DNA methylation quantification ultra kit, the colorimetric version of quantifying methylated DNA.
Global DNA hypomethylation is likely caused by methyl-deficiency due to a variety of environmental influences and has been proposed as a molecular marker in multiple biological processes such as cancer.
It is well demonstrated that the decrease in global DNA methylation is one of the most important characteristics of cancer.
Therefore the quantification of global methylation in cancer cells provides useful information for detection and analysis of this disease.
Epigentek said offering a kit-based method for such quantification is an improvement over conventional methods (for example, mass spectrometry, enzymatic degradation and analysis, and immunohistostaining) and overcomes limitations such as the need for special equipment, long protocols, low sensitivity, and radioactivity.
The Supersense kit is a fluorescence-based assay that can be completed in three easy-to-follow steps within 2.5 hours via a convenient 96-well plate format.
The kit is sensitive with the detection limit as low as 50pg of methylated DNA, suitable for quantifying methylated DNA using a small amount of DNA samples.
Epigentek also simultaneously released a fluorometric version of its Epiquik DNA methyltransferase activity/inhibition assay kit.
