Olympus has introduced accessories that enhance the capabilities of its Fluoview FV1000-MPE multiphoton system.
The developments include a new Laser Sharing System, Dual-Port SIM Scanner, and Multi-Point SIM scanner software.
The Laser Sharing System lets researchers use a single Spectra-Physics Mai Tai Deepsee laser system to perform imaging experiments on two separate Olympus multiphoton systems, providing unprecedented flexibility and cost savings for core facilities and scientists that want to install multiple two-photon instruments, or systems with varying capabilities.
Both systems can be used simultaneously with the laser if using the same wavelength of light.
The Laser Sharing System eliminates the need for a second laser by optically redirecting laser light to accommodate a second multiphoton system.
The Dual-Port SIM (Simultaneous) scanner offers unprecedented flexibility to users whose experimental protocols require photobleaching with both visible and infrared (IR) light.
The scanner lets the user do both without changing any optics or rehooking fibres, eliminating laser alignment issues, saving time and cutting down on hassles that can interfere with imaging.
The new SIM scanner software enables the researcher to rapidly stimulate multi-point areas commonly needed in neuronal spine research.
The software, with its dedicated neuroscience application, enables the user to control the scanner, laser and dataset for stimulation applications used in uncaging or channel rhodopsin experiments.
The SIM scanner software visualises the signal, not only from the confocal photomultiplier tube (PMT), but also from electrophysiology patch-clamp equipment through a new interface unit.