Malvern has published a presentation on its website about its Viscotek TDAmax multiple detection size-exclusion-chromatography system.
The presentation discusses how the system enables protein scientists to determine the degree of oligomerisation, aggregation, conformation and conjugation in protein samples.
TDAmax delivers efficient measurement of absolute molecular weight and molecular size, as well as information about protein structure that is not available using more traditional systems.
The system incorporates refractive index, light-scattering and viscometer detectors, processing all three signals together at each data slice (5 Hz).
By incorporating a combination of low-angle (7deg) and right-angle (90deg) light-scattering detectors, it can measure the absolute molecular weight of a sample directly as it elutes from the column, without extrapolation or any additional modelling.
Molecular weight, intrinsic viscosity and molecular size are determined across the entire distribution, with software enabling the results to be visualised graphically for ease of interpretation.
The addition of a fourth detector, a UV or Diode Array UV, adds in compositional analysis.
