Premier Biosoft International has announced the release of Beacon Designer Version 7.7, which comes with design support for high-resolution melting-analysis (HRMA) primers.
HRMA is an upcoming post-PCR analysis method, which is suitable for SNP genotyping for the identification of variants prior to sequencing.
Beacon Designer uses sophisticated algorithms to guarantee analysis by high-resolution melting.
HRMA primers are designed flanking the SNP of interest to generate the shortest possible amplicons with detectable melting temperature variation.
The primers are designed avoiding template secondary structures, ensuring efficient primer extension, according to the company.
Beacon Designer enables the analysis of predesigned or published primers.
It also supports the design of oligos for SYBR Green, Taqman, LNA-spiked Taqman, Methylight, molecular beacon, Scorpion, Nasba and Fret assays.
