Agilent Technologies has announced the availability of the Brilliant III ultra-fast QPCR and QRT-PCR master-mix kits.
The new kits are claimed to offer a reduced cycling time and good sensitivity without compromising the accuracy and reproducibility of nucleic-acid quantification.
The Brilliant III ultra-fast QPCR and QRT-PCR master-mix reagents are designed to provide fast cycling times on any real-time PCR instrument.
These reagents feature a Taq, delivering a faster extension rate, combined with hot-start technology to minimise non-specific amplification.
The ultra-fast reagents allow the completion of real-time experiments in less than 40 minutes.
The kits enable researchers to perform a variety of applications, including gene expression analysis, pathogen detection and SNP genotyping.
The reagents are validated across a range of fast, real-time PCR systems and across various templates and targets.
Earlier threshold cycle detection provides reliable quantification across the entire range of template concentrations, including high and low concentrations, according to the company.
The Brilliant III ultra-fast QPCR and QRT-PCR master mixes are Agilent's third generation of real-time PCR reagents to be commercialised.