Alphabiotech has released the MPCS Plugmaker from Emerald Biosystems.
Based on lab-on-a-chip micro-fluidics, the Plugmaker features: 5-10nL/experiment sample volume; up to 800 experiments/4uL protein; no dead volume; enables systematic assessment of optimal protein crystallisation slot through optimisation screens; crystals directly amenable to X-ray diffraction experiments with standard equipment; crystals can be readily extracted for traditional cryo-preservation; and low cost per experiment.
Optimal protein crystallisation growth conditions can be identified by systematic variation of one or two chemical components.
MPCS fine-gradient screening via automated on-chip formulation is an optimisation tool that allows thorough interrogation of specific regions of crystallisation phase space.
Sparse matrix screening is achieved with the Plug Maker using the Hybrid Mode.
In the Hybrid Mode, a pre-formed cartridge from a 96-well plate, containing a series of precipitants, is generated and combined with the protein sample inside the Crystalcard.
Crystals are easily extracted from the Crystalcards by peeling off the back and looping the crystals.
