Single Step filter vials from Thompson Instrument are described as a simple solution to sample blockage problems in HPLC, LC-MS and similar techniques, and are available in the UK from Asynt.
Automated analytical systems such as high-performance liquid chromatography (HPLC), gas chromatography (GC) and hybrid techniques such as liquid chromatography-mass spectrometry (LC-MS) can suffer downtime and loss of productivity due to blockage of the autosampler syringe or the analytical column, particularly when samples contain particulate debris such as protein material or catalyst residues.
By incorporating the necessary filter into a standard size autosampler vial, Single Step filter vials (patent pending) replace the usual syringe, syringe filter, HPLC vial and vial cap, and make the filtration process a single step, saving time and reducing the risk of sample loss or contamination.
Filtration is carried out by a 0.45-micron porosity PTFE membrane in the base of a plunger that slides into the body of the vial.
The sample (450ul to the fill line) is placed in the vial, and the plunger then pushed down into the vial, forcing the sample through the filter.
The clear filtrate is then available above the filter and the vial can be sealed with the ready-slit septum cap.
The vial can then be loaded into any standard autosampler, and Single Step filter vials are compatible with all major brands.
