Sera Laboratories' Splitkits cell dissociation system is designed for use in both serum-based and serum-free methodologies in lab cell-culture and biopharmaceutical manufacturing, and flow cytometry.
Developed using a vegetable-based component rather than trypsin, Splitkits avoid any risk of contamination with either animal or human viruses or bacteria, and are suitable for a variety of cell-culture systems.
Independent studies conducted in both research and biopharmaceutical laboratories have shown that Splitkits encourage faster cell dissociation than trypsin.
Greater control of the dissociation process produces homogeneous cell suspensions with minimal batch-to-batch variation.
Sera Laboratories said the gentle action of the Splitkits vegetable-based enzyme gives higher cell yields and better cell growth recovery following detachment.
In addition, surface epitopes and cell membranes remain unaltered and fully functional, making the kits ideal for use in flow cytometry.
Each Splitkit is composed of vegetable-based enzymatic reagent (Splitcells), Antisplitcells and Splitcells buffer, to make a flexible cell dissociation system that can perform at ambient temperature.
There are three different formats in the Splitkits range, each being optimised for use with specific cell types: Splitkit Strong, for strongly adherent and bioreactor-cultured cells; Splitkit Moderate for moderately adherent cells; and Splitkit Mild for mildly adherent cells.
