Shimadzu has partnered with Covalx to offer the Axima Megatof, an integrated Maldi solution for ultra-high-mass applications.
It can be used for protein complex characterisation, therapeutic protein aggregates, antibody-antigen interactions, polymer analysis and high-mass bioimaging.
The high-sensitivity Megatof, which combines a high-performance Shimadzu linear Maldi TOF mass spectrometer with a Covalx high-mass detection system, detects macromolecules up to 1,500kDa.
The MegaTOF stabilises intact protein complexes during analysis with the combination of Covalx's high-mass system, offering nM detection of macromolecules up to 1,500kDa, and Maldi stabilisation kits, which provide cross-linking reagents and buffers for fast and specific stabilisation of protein interactions.
These reagents contain cocktails of cross-linkers with different spacer lengths that can covalently bind specific proteins with a high degree of efficiency, even in contaminated and unpurified samples.
Additionally, the Megatof can detect protein interactions, such as aggregation, directly from formulation mixtures.
Megatof is also equipped with a conventional detector for low-mass samples (less than 20kDa).
Existing Shimadzu Axima Maldi systems can be retrofitted with Covalx's HM2 or HM2 Tuvo high-mass system.
