Softgenetics has launched version 2.0 of its Nextgene software for the analysis of data from the Roche GS, Illumina GA and Applied Biosystems Solid sequencing platforms.
This latest version incorporates a number of new features, functionalities and efficiencies requested by biologists, and compatibility with GRCh37.
These include viewing and filtering capabilities including a variant confidence scoring tool that scores each found variant by four different factors, providing a useful method to filter out low confidence calls.
Another addition is the variant comparison tool, which compares up to 10 patients or samples, listing the similarities and differences between samples in a colour-coded interactive report.
Other additions include: compatibility with GRCh37 in both base and colour space for the Solid system, the ability to list more than 200 million variants, various filtering and sorting options and search capabilities.
As well as being available in the processing software, these capabilities are also incorporated into the latest Nextgene Viewer.
The processing capabilities of Nextgene have also been upgraded.
The software now includes the ability to remove duplicate reads, enhanced facility for paired end analysis, improved alignment algorithms for detection of Indels, automated analysis parameter setting based upon on the particular dataset being analysed, as well as increased alignment speed with a new seed filter.
Nextgene now also provides support for Roche mate Pair SFF files.
