Oxford Expression Technologies has launched Poet3, a baculovirus transfer plasmid for improved expression of glycosylated, secreted and membrane-targeted proteins.
Poet3 is said to combine the flexibility and convenience offered by the Poet1 and Poet2 vectors, with enhanced capabilities that complement improvements in protein expression delivered by the company's Flashbac vectors.
Poet1 and Poet2 are baculovirus transfer plasmids designed for high-level expression of foreign genes under the AcMNPV polyhedrin (polh) promoter.
Poet3 protein expression is driven from the AcMNPV p6.9 promoter, enabling earlier expression compared with the polh promoter used in Poet1 and Poet2.
The recombinant protein is therefore produced at a time during the infection process when the cellular machinery is in a much healthier condition, compared with the late times in infection.
This can give improvements when proteins that require extensive post-translational modifications are produced, such as glycoproteins destined for secretion or membrane insertion.
Poet3 plasmids are also compatible with all baculovirus systems based on homologous recombination and have the same MCS and small size as Poet1 to facilitate easy cloning.
