The Summer 2010 edition of the IFC Application Hotspot e-newsletter from Fluidigm is dedicated to advances in single-cell research and is now available for free download.
The newsletter includes two customer application studies, a recorded video interview with the Genome Insitute of Singapore and a new single-cell gene expression protocol.
The first application study reports on how resolution of cell fate decisions can be revealed by single-cell gene expression analysis from zygote to blastocyst.
Using Dynamic Array Integrated Fluidic Circuits (IFCs), and the mouse pre-implantation embryo as a model, scientists from the Genome Institute of Singapore applied single-cell analysis to study the expression dynamics of 48 genes across mutliple developmental time points.
The described work illustrates the power of single-cell expression analysis to provide insight into developmental mechanisms.
The second application study describes how using Dynamic Array IFCs, scientists at Keio University analysed the expression of various cadherin genes in long-term hematopoietic stem cells (HSCs) and subpopulations of the HSCs isolated from the endosteum to better understand cadherin function as it relates to HSC-niche interactions.
In a video interview, Dr Paul Robson of the Genome Institute of Singapore discusses his latest research on single-cell expression data, which is crucial for a detailed molecular understanding of mammalian development from fertilisation to embryo implantation.
His research has direct impact on clinical applications in the areas of regenerative medicine and assisted reproduction.
