FEI has released a set of software applications that increase the throughput and ease of use of its electron microscopes for biological research.
The four software packages make electron microscopes more useful for life-science researchers involved in structural, cellular and tissue biology as they build the full solution from sample to biological answer.
Dominique Hubert, vice-president at FEI and general manager of the life-sciences division, said: 'Electron microscopy has already played, and will continue to play a key role in the signature discoveries of this century, for instance, in helping to identify the structure-function relationships in biological systems that could lead to improved diagnostics and more effective drugs.
'We are excited to announce a correlative workflow utility that helps to bridge the gap between light microscopy and electron microscopy.
'Now, researchers can use a routine light microscope to locate a feature of interest, and then transfer the sample to an electron microscope, which can be used to easily navigate to the feature and view the cellular ultrastructure.
'Correlative platforms such as this could actually speed the process from research to discovery,' Hubert added.
The software offerings include the correlative navigation utility for the correlation of navigational co-ordinate systems between different types of microscopes, such as optical and electron microscopes.
Investigators can leverage the strengths of each platform, for example, in using the resolving power of electron microscopy to image structures localised by fluorescent tags in a light microscope.
EPU is an automated data collection procedure that facilitates the acquisition of large datasets (from thousands or tens of thousands of nominally identical particles) used to reconstruct high-resolution 3D models with the single particle analysis technique.
Automated Recognition of Geometries, Objects and Segmentations (ARGOS) is a 3D template fitting capability that helps to localise macromolecules in their native cellular context and relate orientation properties of these molecules to their environment.
It combines high-resolution molecular structure information that was determined by single particle analysis with 3D cellular context from tomography.
Extended Slice and View is a 3D reconstruction technique that combines automated serial cross-sectional SEM image acquisition into a virtual 3D volume image of the tissue or cell.
The images may be stitched together from multiple images of the section surface to retain nanometre-scale detail over many micrometre fields of view.
The focused ion beam (FIB) cross sections can be as thin as a few nanometres, providing near-isotropic resolution in the X, Y and Z dimensions.
The technique has the potential to model whole cells and tissues with sufficient resolution to differentiate lipid bilayers.
