EMD Millipore has discussed a new clarification process for high-density cell culture fluids at the 14th Recovery of Biological Products Conference in California, US.
Sarah Hove, manager of process development at EMD Millipore, presented 'Development of a Robust Clarification Process for High-Density Mammalian Cell Culture Processes Using Flocculation Methods and an Optimised Dual-Stage Separation Technology' at the event.
The talk covered an improved process to alleviate the increasingly challenging harvest clarification step.
The development of a dual-stage separation technology in conjunction with flocculation allows for a more robust harvest step across a range of molecules and cell culture fluids for high-productivity cell culture processes.
Richard Pearce, director - purification solutions at EMD Millipore, said: 'Increases in cell culture productivity and cell density have posed challenges during harvest operations with existing centrifugation and depth filter technologies.
'This technology shows that flocculation in conjunction with optimised separation media can eliminate the bottleneck in the harvest step by improving capacity and process consistency,' he added.
The Process Solutions business unit of EMD Millipore also showcased three posters at the conference detailing a mechanistic understanding of cellulosic depth filters, concepts for flow-through purification for viral vaccines, and an alternative filter sizing model.
