Fluidigm has described how its Biomark system and 96.96 Dynamic Array Integrated Fluidic Circuit (IFC) have been used to verify and genotype SNPs in Alaskan populations of chum salmon.
An application poster reveals how the University of Washington has used the Biomark system and 96.96 Dynamic Array IFC for verification and genotyping of SNPs in Alaskan populations of chum salmon.
Next-generation sequencing techniques have increased the reliability of SNPs for studies of population genetics.
Cost effective laboratory procedures at sufficient throughput for SNP verification and genotyping have not always kept pace.
At the same time, the use of SNPs for tackling very difficult population problems has become appealing for fish management and conservation.
The researchers describe the development of new melt curve analysis methods (on four closely related Chum Salmon populations) they have used to rapidly and inexpensively verify putative SNPs through next-generation sequencing.
Using the described methodology, the researchers produce a set of verified SNPs that can now be tested across all chum salmon populations.
Using the Fluidigm 96.96 Dynamic Array IFC on the Fluidigm Biomark system, the researchers were able to simultaneously run 96 samples against 96 SNP genotyping assays for a total of 9,216 genotypes on a single chip.
They concluded that the Fluidigm platform is well suited for high-throughput SNP genotyping and offers a fast and easy workflow that provides timely and cost-effective results.
