The thermo-reversible mountant Cygel from Biostatus allows the high-resolution imaging of live 3D tumour spheroids cultured in low-adherence conditions and without fixation or cytospin centrifugation.
A recent paper by Fredika M Robertson et al, entitled 'Imaging and Analysis of 3D Tumor Spheroids Enriched for a Cancer Cell Phenotype' (J Biomol Screen, 2010, 15:820-829), confirms that Cygel stabilises multi-cellular structures for imaging and characterisation, while retaining cellular viability, morphology and protein expression.
Cygel is optically inert and clear, compatible with fluorescence and permits the delivery of organelle-specific and viability probes.
Objects can be isolated, imaged and then recovered for orthogonal analysis.
Cygel enables advanced microscopic techniques for the live-cell imaging of both mammalian and non-mammalian structures.
